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plate-tool
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Basic simple transfers

This commit is contained in:
Emilia Allison 2023-05-13 11:40:39 -04:00
parent 6bafb0372b
commit 6f56973b07
Signed by: emilia
GPG Key ID: 7A3F8997BFE894E0
2 changed files with 103 additions and 7 deletions
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105
src/data/transfer_region.rs
View File

@ -1,8 +1,20 @@
use super::plate::Plate;
#[derive(Clone, Copy)]
pub enum Region {
Rect((u8,u8),(u8,u8)),
Point(u8,u8)
Point((u8,u8))
}
impl TryFrom<Region> for ((u8,u8),(u8,u8)) {
type Error = &'static str;
fn try_from(region: Region) -> Result<Self, Self::Error> {
if let Region::Rect(c1, c2) = region {
Ok((c1,c2))
} else {
// Should consider returning a degenerate rectangle here instead
Err("Cannot convert this region to a rectangle, it was a point.")
}
}
}
pub struct TransferRegion<'a> {
@ -37,6 +49,72 @@ impl TransferRegion<'_> {
return wells;
} else { panic!("Source region is just a point!") }
}
pub fn get_destination_wells(&self) -> Vec<(u8,u8)> {
let map = self.calculate_map();
let source_wells = self.get_source_wells();
let mut wells = Vec::<(u8,u8)>::new();
for well in source_wells {
if let Some(dest_well) = map(well) {
wells.push(dest_well)
}
}
return wells;
}
pub fn calculate_map(&self) -> Box<dyn Fn((u8,u8)) -> Option<(u8,u8)> + '_> {
let source_wells = self.get_source_wells();
let il_dest = self.interleave_dest.unwrap_or((1,1));
let il_source = self.interleave_source.unwrap_or((1,1));
if il_source.0 == 0 { let il_source = (1,il_source.1); }
if il_source.1 == 0 { let il_source = (il_source.1,il_source.0); }
let source_corners: ((u8,u8),(u8,u8)) = self.source_region.try_into()
.expect("Source region should not be a point");
let (source_ul, _) = standardize_rectangle(&source_corners.0, &source_corners.1);
// This map is not necessarily injective or surjective,
// but we will have these properties in certain cases.
// If the transfer is not a pooling transfer (interleave == 0)
// then we *will* have injectivity.
// First we'll handle ij-pooling transfers, since they're the simplest.
// NOTE: I'm going to say that these transfers always go to the primary corner,
// because I can't envision how an ij-pooling replicate transfer would work.
if il_dest == (0,0) {
return Box::new(move |(i,j)| { // All we're moving here is source_wells
if source_wells.contains(&(i,j)) {
match self.dest_region {
Region::Point((i0, j0)) => Some((i0,j0)),
Region::Rect((i0,j0),_) => Some((i0,j0))
}
} else {
None
}
})
}
// Non-replicate transfers:
if let Region::Point((x,y)) = self.dest_region {
return Box::new(move |(i,j)| {
if source_wells.contains(&(i,j)) {
let il_source = self.interleave_source.unwrap_or((1,1));
Some((
x + i.checked_sub(source_ul.0).expect("Point cannot have been less than UL")
.checked_div(il_source.0.abs() as u8).expect("Source interleave cannot be 0")
.mul(il_dest.0.abs() as u8),
y + j.checked_sub(source_ul.1).expect("Point cannot have been less than UL")
.checked_div(il_source.1.abs() as u8).expect("Source interleave cannot be 0")
.mul(il_dest.1.abs() as u8),
))
} else { None }
})
} else {
return Box::new(move |(_,_)| None)
}
}
pub fn validate(&self) -> Result<(), String> {
// Checks if the region does anything suspect
@ -50,7 +128,7 @@ impl TransferRegion<'_> {
// Easy checks:
match self.source_region {
Region::Point(_, _) => return Err("Source region should not be a point!".to_string()),
Region::Point(_) => return Err("Source region should not be a point!".to_string()),
Region::Rect(c1, c2) => {
// Check if all source wells exist:
if c1.0 == 0 || c1.1 == 0
@ -69,7 +147,7 @@ impl TransferRegion<'_> {
}
// Check that source lengths divide destination lengths
match &self.dest_region {
Region::Point(_,_) => (),
Region::Point(_) => (),
Region::Rect(c1, c2) => {
let dest_diff_i = u8::abs_diff(c1.0, c2.0);
let dest_diff_j = u8::abs_diff(c1.1, c2.1);
@ -105,7 +183,7 @@ fn in_region(pt: (u8,u8), r: &Region) -> bool {
&& pt.1 <= u8::max(c1.1, c2.1)
&& pt.1 >= u8::min(c1.1, c2.1)
},
Region::Point(i, j) => {
Region::Point((i, j)) => {
pt.0 == *i && pt.1 == *j
}
}
@ -121,6 +199,7 @@ fn standardize_rectangle(c1: &(u8,u8), c2: &(u8,u8)) -> ((u8,u8),(u8,u8)) {
#[cfg(debug_assertions)]
use std::fmt;
use std::ops::Mul;
#[cfg(debug_assertions)]
impl fmt::Display for TransferRegion<'_> {
@ -139,6 +218,22 @@ impl fmt::Display for TransferRegion<'_> {
}
source_string.push_str("\n");
}
write!(f, "{}", source_string)
write!(f, "{}", source_string)?;
writeln!(f, "Dest Plate:")?;
let dest_dims = self.dest_plate.size();
let dest_wells = self.get_destination_wells();
let mut dest_string = String::new();
for i in 1..=dest_dims.0 {
for j in 1..=dest_dims.1 {
if dest_wells.contains(&(i,j)) {
dest_string.push_str("x")
} else {
dest_string.push_str("o")
}
}
dest_string.push_str("\n");
}
write!(f, "{}", dest_string)
}
}

5
src/lib.rs
View File

@ -28,10 +28,11 @@ pub fn plate_test() {
source_plate: &source,
source_region: transfer_region::Region::Rect((1,1), (6,6)),
dest_plate: &destination,
dest_region: transfer_region::Region::Rect((1,1), (6,6)),
interleave_source: Some((2,1)),
dest_region: transfer_region::Region::Point((2,2)),
interleave_source: None,
interleave_dest: None
};
println!("{}", transfer);
println!("{:?}", transfer.get_source_wells());
println!("{:?}", transfer.get_destination_wells());
}