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1 changed files with 84 additions and 116 deletions

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@ -131,8 +131,10 @@ impl TransferRegion {
pub fn calculate_map(&self) -> Box<dyn Fn(Well) -> Option<Vec<Well>> + '_> { pub fn calculate_map(&self) -> Box<dyn Fn(Well) -> Option<Vec<Well>> + '_> {
// By validating first, we have a stronger guarantee that // By validating first, we have a stronger guarantee that
// this function will not panic. :) // this function will not panic. :)
// log::debug!("Validating: {:?}", self.validate());
if let Err(msg) = self.validate() { if let Err(msg) = self.validate() {
log::error!("{}\nThis transfer will be empty.", msg); eprintln!("{}", msg);
eprintln!("This transfer will be empty.");
return Box::new(|_| None); return Box::new(|_| None);
} }
@ -202,30 +204,22 @@ impl TransferRegion {
); );
let count = ( let count = (
// How many times can we replicate? // How many times can we replicate?
if il_dest.0.unsigned_abs() == 0 { (1..)
1 .position(|n| {
} else { n * number_used_src_wells.0 * il_dest.0.unsigned_abs()
(1..) - il_dest.0.unsigned_abs()
.position(|n| { + 1
(n * number_used_src_wells.0 * il_dest.0.unsigned_abs()) > d_dims.0
.saturating_sub(il_dest.0.unsigned_abs()) })
+ 1 .unwrap() as u8,
> d_dims.0 (1..)
}) .position(|n| {
.unwrap() as u8 n * number_used_src_wells.1 * il_dest.1.unsigned_abs()
}, - il_dest.1.unsigned_abs()
if il_dest.1.unsigned_abs() == 0 { + 1
1 > d_dims.1
} else { })
(1..) .unwrap() as u8,
.position(|n| {
(n * number_used_src_wells.1 * il_dest.1.unsigned_abs())
.saturating_sub(il_dest.1.unsigned_abs())
+ 1
> d_dims.1
})
.unwrap() as u8
},
); );
let i = i let i = i
.saturating_sub(s_ul.row) .saturating_sub(s_ul.row)
@ -233,34 +227,30 @@ impl TransferRegion {
let j = j let j = j
.saturating_sub(s_ul.col) .saturating_sub(s_ul.col)
.saturating_div(il_source.1.unsigned_abs()); .saturating_div(il_source.1.unsigned_abs());
let checked_il_dest = (u8::max(il_dest.0.unsigned_abs(), 1u8),
u8::max(il_dest.1.unsigned_abs(), 1u8));
let row_modulus = number_used_src_wells.0 * checked_il_dest.0;
let column_modulus = number_used_src_wells.1 * checked_il_dest.1;
Some( Some(
possible_destination_wells possible_destination_wells
.into_iter() .into_iter()
.filter(|Well { row: x, .. }| { .filter(|Well { row: x , ..}| {
x.checked_sub(d_ul.row).unwrap() x.checked_sub(d_ul.row).unwrap()
% row_modulus // Counter along x % (number_used_src_wells.0 * il_dest.0.unsigned_abs()) // Counter along x
== (il_dest.0.unsigned_abs() *i) == (il_dest.0.unsigned_abs() *i)
% row_modulus % (number_used_src_wells.0 * il_dest.0.unsigned_abs())
}) })
.filter(|Well { col: y, .. }| { .filter(|Well { col: y, .. }| {
y.checked_sub(d_ul.col).unwrap() y.checked_sub(d_ul.col).unwrap()
% column_modulus // Counter along u % (number_used_src_wells.1 * il_dest.1.unsigned_abs()) // Counter along u
== (il_dest.1.unsigned_abs() *j) == (il_dest.1.unsigned_abs() *j)
% column_modulus % (number_used_src_wells.1 * il_dest.1.unsigned_abs())
}) })
.filter(|Well { row: x, col: y }| { .filter(|Well { row: x, col: y }| {
// How many times have we replicated? < How many are we allowed // How many times have we replicated? < How many are we allowed
// to replicate? // to replicate?
x.checked_sub(d_ul.row).unwrap().div_euclid( x.checked_sub(d_ul.row).unwrap().div_euclid(
row_modulus number_used_src_wells.0 * il_dest.0.unsigned_abs(),
) < count.0 ) < count.0
&& y.checked_sub(d_ul.col).unwrap().div_euclid( && y.checked_sub(d_ul.col).unwrap().div_euclid(
column_modulus number_used_src_wells.1 * il_dest.1.unsigned_abs(),
) < count.1 ) < count.1
}) })
.collect(), .collect(),
@ -318,15 +308,11 @@ impl TransferRegion {
// log::debug!("s1.1: {}, max.1: {}", s1.1, source_max.1); // log::debug!("s1.1: {}, max.1: {}", s1.1, source_max.1);
return Err("Source region is out-of-bounds! (Too wide)"); return Err("Source region is out-of-bounds! (Too wide)");
} }
if il_dest == (0,0) {
return Err("Refusing to pool both dimensions in a rectangular transfer!\nPlease select a point in the destination plate.")
}
} }
Region::Custom(_) => return Ok(()), Region::Custom(_) => return Ok(()),
} }
if il_source.0 == 0 || il_source.1 == 0 { if il_source.0 == 0 || il_dest.1 == 0 {
return Err("Source interleave cannot be zero!"); return Err("Source interleave cannot be zero!");
} }
@ -423,85 +409,85 @@ mod tests {
let transfer1 = TransferRegion { let transfer1 = TransferRegion {
source_plate: source, source_plate: source,
source_region: Region::Rect(Well { row: 1, col: 1 }, Well { row: 1, col: 1 }), source_region: Region::Rect(Well{ row: 1, col: 1}, Well { row: 1, col: 1 }),
dest_plate: destination, dest_plate: destination,
dest_region: Region::Point(Well { row: 3, col: 3 }), dest_region: Region::Point(Well {row: 3, col: 3}),
interleave_source: (1, 1), interleave_source: (1, 1),
interleave_dest: (1, 1), interleave_dest: (1, 1),
}; };
let transfer1_map = transfer1.calculate_map(); let transfer1_map = transfer1.calculate_map();
assert_eq!( assert_eq!(
transfer1_map(Well { row: 1, col: 1 }), transfer1_map(Well {row: 1, col: 1}),
Some(vec! {Well {row: 3, col: 3}}), Some(vec! {Well {row: 3, col: 3}}),
"Failed basic shift transfer 1" "Failed basic shift transfer 1"
); );
assert_eq!( assert_eq!(
transfer1_map(Well { row: 1, col: 2 }), transfer1_map(Well{ row: 1, col: 2}),
Some(vec! {Well{ row: 3, col: 4}}), Some(vec! {Well{ row: 3, col: 4}}),
"Failed basic shift transfer 2" "Failed basic shift transfer 2"
); );
assert_eq!( assert_eq!(
transfer1_map(Well { row: 2, col: 2 }), transfer1_map(Well{ row: 2, col: 2}),
Some(vec! {Well{ row: 4, col: 4}}), Some(vec! {Well{ row: 4, col: 4}}),
"Failed basic shift transfer 3" "Failed basic shift transfer 3"
); );
let transfer2 = TransferRegion { let transfer2 = TransferRegion {
source_plate: source, source_plate: source,
source_region: Region::Rect(Well { row: 1, col: 1 }, Well { row: 3, col: 3 }), source_region: Region::Rect(Well{ row: 1, col: 1}, Well{ row: 3, col: 3}),
dest_plate: destination, dest_plate: destination,
dest_region: Region::Point(Well { row: 3, col: 3 }), dest_region: Region::Point(Well{ row: 3, col: 3}),
interleave_source: (2, 2), interleave_source: (2, 2),
interleave_dest: (1, 1), interleave_dest: (1, 1),
}; };
let transfer2_map = transfer2.calculate_map(); let transfer2_map = transfer2.calculate_map();
assert_eq!( assert_eq!(
transfer2_map(Well { row: 1, col: 1 }), transfer2_map(Well{ row: 1, col: 1}),
Some(vec! {Well{ row: 3, col: 3}}), Some(vec! {Well{ row: 3, col: 3}}),
"Failed source interleave, type simple 1" "Failed source interleave, type simple 1"
); );
assert_eq!( assert_eq!(
transfer2_map(Well { row: 1, col: 2 }), transfer2_map(Well{ row: 1, col: 2}),
None, None,
"Failed source interleave, type simple 2" "Failed source interleave, type simple 2"
); );
assert_eq!( assert_eq!(
transfer2_map(Well { row: 2, col: 2 }), transfer2_map(Well{ row: 2, col: 2}),
None, None,
"Failed source interleave, type simple 3" "Failed source interleave, type simple 3"
); );
assert_eq!( assert_eq!(
transfer2_map(Well { row: 3, col: 3 }), transfer2_map(Well{ row: 3, col: 3}),
Some(vec! {Well{ row: 4, col: 4}}), Some(vec! {Well{ row: 4, col: 4}}),
"Failed source interleave, type simple 4" "Failed source interleave, type simple 4"
); );
let transfer3 = TransferRegion { let transfer3 = TransferRegion {
source_plate: source, source_plate: source,
source_region: Region::Rect(Well { row: 1, col: 1 }, Well { row: 3, col: 3 }), source_region: Region::Rect(Well{ row: 1, col: 1}, Well{ row: 3, col: 3}),
dest_plate: destination, dest_plate: destination,
dest_region: Region::Point(Well { row: 3, col: 3 }), dest_region: Region::Point(Well{ row: 3, col: 3}),
interleave_source: (1, 1), interleave_source: (1, 1),
interleave_dest: (2, 3), interleave_dest: (2, 3),
}; };
let transfer3_map = transfer3.calculate_map(); let transfer3_map = transfer3.calculate_map();
assert_eq!( assert_eq!(
transfer3_map(Well { row: 1, col: 1 }), transfer3_map(Well{ row: 1, col: 1}),
Some(vec! {Well{ row: 3, col: 3}}), Some(vec! {Well{ row: 3, col: 3}}),
"Failed destination interleave, type simple 1" "Failed destination interleave, type simple 1"
); );
assert_eq!( assert_eq!(
transfer3_map(Well { row: 2, col: 1 }), transfer3_map(Well{ row: 2, col: 1}),
Some(vec! {Well{ row: 5, col: 3}}), Some(vec! {Well{ row: 5, col: 3}}),
"Failed destination interleave, type simple 2" "Failed destination interleave, type simple 2"
); );
assert_eq!( assert_eq!(
transfer3_map(Well { row: 1, col: 2 }), transfer3_map(Well{ row: 1, col: 2}),
Some(vec! {Well{ row: 3, col: 6}}), Some(vec! {Well{ row: 3, col: 6}}),
"Failed destination interleave, type simple 3" "Failed destination interleave, type simple 3"
); );
assert_eq!( assert_eq!(
transfer3_map(Well { row: 2, col: 2 }), transfer3_map(Well{ row: 2, col: 2}),
Some(vec! {Well{ row: 5, col: 6}}), Some(vec! {Well{ row: 5, col: 6}}),
"Failed destination interleave, type simple 4" "Failed destination interleave, type simple 4"
); );
@ -514,33 +500,29 @@ mod tests {
let transfer1 = TransferRegion { let transfer1 = TransferRegion {
source_plate: source, source_plate: source,
source_region: Region::Rect(Well { row: 1, col: 1 }, Well { row: 2, col: 2 }), source_region: Region::Rect(Well{ row: 1, col: 1}, Well{ row: 2, col: 2}),
dest_plate: destination, dest_plate: destination,
dest_region: Region::Rect(Well { row: 2, col: 2 }, Well { row: 11, col: 11 }), dest_region: Region::Rect(Well{ row: 2, col: 2}, Well{row:11, col:11}),
interleave_source: (1, 1), interleave_source: (1, 1),
interleave_dest: (3, 3), interleave_dest: (3, 3),
}; };
let transfer1_map = transfer1.calculate_map(); let transfer1_map = transfer1.calculate_map();
assert_eq!( assert_eq!(
transfer1_map(Well { row: 1, col: 1 }), transfer1_map(Well{ row: 1, col: 1}),
Some( Some(vec! {Well{ row: 2, col: 2}, Well{ row: 2, col: 8}, Well{ row: 8, col: 2}, Well{ row: 8, col: 8}}),
vec! {Well{ row: 2, col: 2}, Well{ row: 2, col: 8}, Well{ row: 8, col: 2}, Well{ row: 8, col: 8}}
),
"Failed type replicate 1" "Failed type replicate 1"
); );
assert_eq!( assert_eq!(
transfer1_map(Well { row: 2, col: 1 }), transfer1_map(Well{ row: 2, col: 1}),
Some( Some(vec! {Well{ row: 5, col: 2}, Well{ row: 5, col: 8}, Well{ row: 11, col: 2}, Well{ row: 11, col: 8}}),
vec! {Well{ row: 5, col: 2}, Well{ row: 5, col: 8}, Well{ row: 11, col: 2}, Well{ row: 11, col: 8}}
),
"Failed type replicate 1" "Failed type replicate 1"
); );
let transfer2 = TransferRegion { let transfer2 = TransferRegion {
source_plate: Plate::new(PlateType::Source, PlateFormat::W384), source_plate: Plate::new(PlateType::Source, PlateFormat::W384),
dest_plate: Plate::new(PlateType::Destination, PlateFormat::W384), dest_plate: Plate::new(PlateType::Destination, PlateFormat::W384),
source_region: Region::Rect(Well { row: 1, col: 1 }, Well { row: 2, col: 3 }), source_region: Region::Rect(Well{ row: 1, col: 1}, Well{ row: 2, col: 3}),
dest_region: Region::Rect(Well { row: 2, col: 2 }, Well { row: 11, col: 16 }), dest_region: Region::Rect(Well{ row: 2, col: 2}, Well{ row: 11, col: 16}),
interleave_source: (1, 1), interleave_source: (1, 1),
interleave_dest: (2, 2), interleave_dest: (2, 2),
}; };
@ -548,43 +530,36 @@ mod tests {
let transfer2_dest = transfer2.get_destination_wells(); let transfer2_dest = transfer2.get_destination_wells();
assert_eq!( assert_eq!(
transfer2_source, transfer2_source,
vec![ vec![Well{ row: 1, col: 1}, Well{ row: 1, col: 2}, Well{ row: 1, col: 3}, Well{ row: 2, col: 1}, Well{ row: 2, col: 2}, Well{ row: 2, col: 3}],
Well { row: 1, col: 1 },
Well { row: 1, col: 2 },
Well { row: 1, col: 3 },
Well { row: 2, col: 1 },
Well { row: 2, col: 2 },
Well { row: 2, col: 3 }
],
"Failed type replicate 2 source" "Failed type replicate 2 source"
); );
assert_eq!( assert_eq!(
transfer2_dest, transfer2_dest,
vec![ vec![
Well { row: 2, col: 2 }, Well{ row: 2, col: 2},
Well { row: 2, col: 8 }, Well{ row: 2, col: 8},
Well { row: 6, col: 2 }, Well{ row: 6, col: 2},
Well { row: 6, col: 8 }, Well{ row: 6, col: 8},
Well { row: 2, col: 4 }, Well{ row: 2, col: 4},
Well { row: 2, col: 10 }, Well{ row: 2, col: 10},
Well { row: 6, col: 4 }, Well{ row: 6, col: 4},
Well { row: 6, col: 10 }, Well{ row: 6, col: 10},
Well { row: 2, col: 6 }, Well{ row: 2, col: 6},
Well { row: 2, col: 12 }, Well{ row: 2, col: 12},
Well { row: 6, col: 6 }, Well{ row: 6, col: 6},
Well { row: 6, col: 12 }, Well{ row: 6, col: 12},
Well { row: 4, col: 2 }, Well{ row: 4, col: 2},
Well { row: 4, col: 8 }, Well{ row: 4, col: 8},
Well { row: 8, col: 2 }, Well{ row: 8, col: 2},
Well { row: 8, col: 8 }, Well{ row: 8, col: 8},
Well { row: 4, col: 4 }, Well{ row: 4, col: 4},
Well { row: 4, col: 10 }, Well{ row: 4, col: 10},
Well { row: 8, col: 4 }, Well{ row: 8, col: 4},
Well { row: 8, col: 10 }, Well{ row: 8, col: 10},
Well { row: 4, col: 6 }, Well{ row: 4, col: 6},
Well { row: 4, col: 12 }, Well{ row: 4, col: 12},
Well { row: 8, col: 6 }, Well{ row: 8, col: 6},
Well { row: 8, col: 12 } Well{ row: 8, col: 12}
], ],
"Failed type replicate 2 destination" "Failed type replicate 2 destination"
); );
@ -596,8 +571,8 @@ mod tests {
let transfer1 = TransferRegion { let transfer1 = TransferRegion {
source_plate: Plate::new(PlateType::Source, PlateFormat::W384), source_plate: Plate::new(PlateType::Source, PlateFormat::W384),
dest_plate: Plate::new(PlateType::Destination, PlateFormat::W384), dest_plate: Plate::new(PlateType::Destination, PlateFormat::W384),
source_region: Region::Rect(Well { row: 1, col: 4 }, Well { row: 3, col: 7 }), source_region: Region::Rect(Well{ row: 1, col: 4}, Well{ row: 3, col: 7}),
dest_region: Region::Point(Well { row: 1, col: 9 }), dest_region: Region::Point(Well{ row: 1, col: 9}),
interleave_source: (1, 1), interleave_source: (1, 1),
interleave_dest: (0, 2), interleave_dest: (0, 2),
}; };
@ -607,24 +582,17 @@ mod tests {
// Skipping source check---it's just 12 wells. // Skipping source check---it's just 12 wells.
assert_eq!( assert_eq!(
transfer1_dest, transfer1_dest,
vec![ vec![Well{ row: 1, col: 9}, Well{ row: 1, col: 11}, Well{ row: 1, col: 13}, Well{ row: 1, col: 15}].into_iter().collect(),
Well { row: 1, col: 9 },
Well { row: 1, col: 11 },
Well { row: 1, col: 13 },
Well { row: 1, col: 15 }
]
.into_iter()
.collect(),
"Failed type pool 1 dest" "Failed type pool 1 dest"
); );
assert_eq!( assert_eq!(
transfer1_map(Well { row: 2, col: 6 }), transfer1_map(Well{ row: 2, col: 6}),
Some(vec![Well { row: 1, col: 13 }]), Some(vec![Well{ row: 1, col: 13}]),
"Failed type pool 1 map 1" "Failed type pool 1 map 1"
); );
assert_eq!( assert_eq!(
transfer1_map(Well { row: 3, col: 7 }), transfer1_map(Well{ row: 3, col: 7}),
Some(vec![Well { row: 1, col: 15 }]), Some(vec![Well{ row: 1, col: 15}]),
"Failed type pool 1 map 2" "Failed type pool 1 map 2"
); );
} }